Part:BBa_K3278000:Experience

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3278000

In this part, 488-nm blue light was used to induce mEGFP expression and the light intensity was 9.6uW. To examine the dynamics of mEGFP expression, different illuminating durations (6_h, 12_h, 24_h, 30_h, 36_h) was used in our experiment. Unless stated otherwise, all experiments were carried out in E. coli BL21 strain with in M63 or SOC medium plus 50 μg mL−1 kanamycin. The detailed protocol was listed at the bottom of this part. Results are analyzed in both visualized micrographs and fitted line graphs with the average fluorescence intensity (F.I./a.u.) as the standard, showing the approximate and accurate tendency respectively.

User Reviews

UNIQd208409d6405200a-partinfo-00000000-QINU UNIQd208409d6405200a-partinfo-00000001-QINU