Part:BBa_K3278000:Experience

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Applications of BBa_K3278000

In this part, 488-nm blue light was used to induce mEGFP expression and the light intensity was 9.6uW. To examine the dynamics of mEGFP expression, different illuminating durations (6_h, 12_h, 24_h, 30_h, 36_h) was used in our experiment. Unless stated otherwise, all experiments were carried out in E. coli BL21 strain with in M63 or SOC medium plus 50 μg mL−1 kanamycin. The detailed protocol was listed at the bottom of this part. Results are analyzed in both visualized micrographs and fitted line graphs with the average fluorescence intensity (F.I./a.u.) as the standard, showing the approximate and accurate tendency respectively.

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